Recombinant
RabMAb

Recombinant Anti-WAPL/FOE antibody [EPR3407(2)] - BSA and Azide free (ab232016)

Overview

  • Product name

    Anti-WAPL/FOE antibody [EPR3407(2)] - BSA and Azide free
    See all WAPL/FOE primary antibodies
  • Description

    Rabbit monoclonal [EPR3407(2)] to WAPL/FOE - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human WAPL/FOE aa 50-150. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    ab232016 is the carrier-free version of ab109537 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232016 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.  This product was previously labelled as WAPL

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity

    Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
  • Sequence similarities

    Contains 1 WAPL domain.
  • Cellular localization

    Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • FOE antibody
    • Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
    • Friend of EBNA2 protein antibody
    • KIAA0261 antibody
    • OTTHUMP00000020004 antibody
    • WAPAL antibody
    • WAPL antibody
    • WAPL_HUMAN antibody
    • Wings apart like homolog antibody
    • Wings apart like homolog (Drosophila) antibody
    • Wings apart-like protein homolog antibody
    see all

Images

  • ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109537).

References

ab232016 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232016.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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