Key features and details
- Rabbit polyclonal to WAPL/FOE - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-WAPL/FOE antibody - N-terminal
See all WAPL/FOE primary antibodies
DescriptionRabbit polyclonal to WAPL/FOE - N-terminal
At least four isoforms of WAPL/FOE are known to exist.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human WAPL/FOE (N terminal). The exact sequence is proprietary. 18 amino acid peptide.
Database link: Q7Z5K2
- A20 cell lysate and rat heart tissue.
This product was previously labelled as WAPL
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).|
FunctionRegulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Tissue specificityIsoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
Sequence similaritiesContains 1 WAPL domain.
Cellular localizationNucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
- Information by UniProt
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
Lane 1 : Anti-WAPL/FOE antibody - N-terminal (ab191836) at 1 µg
Lane 2 : Anti-WAPL/FOE antibody - N-terminal (ab191836) at 2 µg
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of rat heart tissue labeling WAPL/FOE with ab191836 at 20 µg/ml
Immunohistochemical analysis of rat heart tissue labeling WAPL/FOE with ab191836 at 5 µg/ml.
ab191836 has not yet been referenced specifically in any publications.