Overview

  • Product name

    Anti-WAPL/FOE antibody - N-terminal
    See all WAPL/FOE primary antibodies
  • Description

    Rabbit polyclonal to WAPL/FOE - N-terminal
  • Host species

    Rabbit
  • Specificity

    At least four isoforms of WAPL/FOE are known to exist.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WAPL/FOE (N terminal). The exact sequence is proprietary. 18 amino acid peptide.
    Database link: Q7Z5K2

  • Positive control

    • A20 cell lysate and rat heart tissue.
  • General notes

     This product was previously labelled as WAPL

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).

Target

  • Function

    Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity

    Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
  • Sequence similarities

    Contains 1 WAPL domain.
  • Cellular localization

    Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • FOE antibody
    • Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
    • Friend of EBNA2 protein antibody
    • KIAA0261 antibody
    • OTTHUMP00000020004 antibody
    • WAPAL antibody
    • WAPL antibody
    • WAPL_HUMAN antibody
    • Wings apart like homolog antibody
    • Wings apart like homolog (Drosophila) antibody
    • Wings apart-like protein homolog antibody
    see all

Images

  • Lane 1 : Anti-WAPL/FOE antibody - N-terminal (ab191836) at 1 µg
    Lane 2 : Anti-WAPL/FOE antibody - N-terminal (ab191836) at 2 µg

    All lanes : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of rat heart tissue labeling WAPL/FOE with ab191836 at 20 µg/ml

  • Immunohistochemical analysis of rat heart tissue labeling WAPL/FOE with ab191836 at 5 µg/ml.

References

ab191836 has not yet been referenced specifically in any publications.

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