Anti-Warp antibody (ab231030)
Key features and details
- Rabbit polyclonal to Warp
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Warp antibody -
Description
Rabbit polyclonal to Warp -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Warp aa 1-300. Expressed in E. coli.
Database link: Q6PCB0 -
Positive control
- WB: Human skin cancer, stomach and stomach cancer tissue. Liver tissue. WB: Rat testis lysate; recombinant human Warp protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 47 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 47 kDa. |
Target
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Function
Promotes matrix assembly. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 1 VWFA domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 64856 Human
- Entrez Gene: 298683 Rat
- Omim: 611901 Human
- SwissProt: Q6PCB0 Human
- SwissProt: Q642A6 Rat
- Unigene: 449009 Human
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Alternative names
- von Willebrand factor A domain-containing protein 1 antibody
- VWA1 antibody
- VWA1_HUMAN antibody
- WARP antibody
Images
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Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for Warp with ab231030 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Warp with ab231030 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for Warp with ab231030 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Warp with ab231030 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Warp antibody (ab231030) at 2 µg/ml + Rat testis tissue lysate
Secondary
HRP-linked Guinea Pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 47 kDa -
Anti-Warp antibody (ab231030) at 2 µg/ml + Recombinant human Warp protein
Secondary
HRP-linked Guinea Pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 47 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231030 has not yet been referenced specifically in any publications.