Anti-WASF2/WAVE2 antibody (ab204376)
Key features and details
- Rabbit polyclonal to WASF2/WAVE2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-WASF2/WAVE2 antibody -
Description
Rabbit polyclonal to WASF2/WAVE2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human WASF2/WAVE2 aa 250-350.
Database link: Q9Y6W5 -
Positive control
- IHC-P: Human esophagus, placenta, endometrium, Fallopian tube tissue; ICC: U-2 OS whole cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
-
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. -
Tissue specificity
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. -
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain. -
Domain
Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. At the interface between the lamellipodial actin meshwork and the membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10163 Human
- Entrez Gene: 242687 Mouse
- Omim: 605875 Human
- SwissProt: Q9Y6W5 Human
- SwissProt: Q8BH43 Mouse
- Unigene: 469244 Human
- Unigene: 590909 Human
- Unigene: 23566 Mouse
-
Alternative names
- DICTYOSTELIUM antibody
- dJ393P12.2 antibody
- IMD2 antibody
see all
Images
-
Immunohistochemical analysis of human esophagus tissue labeling WASF2/WAVE2 in the cytoplasm of squamous epithelial cells with ab204376 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling WASF2/WAVE2 in the plasma membrane (green) with ab204376 at 2 µg/ml.
-
Immunohistochemical analysis of human Fallopian tube tissue labeling WASF2/WAVE2 in the cytoplasm of glandular cells with ab204376 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human endometrium tissue labeling WASF2/WAVE2 in the cytoplasm of glandular cells with ab204376 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human placenta tissue labeling WASF2/WAVE2 in the cytoplasm of endothelial cells with ab204376 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human skeletal muscle tissue labeling WASF2/WAVE2 in myocytes with ab204376 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab204376 has not yet been referenced specifically in any publications.