Anti-WASF2/WAVE2 antibody (ab219812)
Key features and details
- Rabbit polyclonal to WASF2/WAVE2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WASF2/WAVE2 antibody
See all WASF2/WAVE2 primary antibodies -
Description
Rabbit polyclonal to WASF2/WAVE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human WASF2/WAVE2 aa 125-174.
Sequence:NTCDTP PPLNNLTPYR DDGKEALKFY TDPSYFFDLW KEKMLQDTKD IMKE
Database link: Q9Y6W5 -
Positive control
- WB: K562 cell lysate. IHC-P: Human lung tissue.
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General notes
This product was previously labelled as WASF2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab219812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 54 kDa. | |
| IHC-P | 1/50. |
Target
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Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. -
Tissue specificity
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. -
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain. -
Domain
Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. At the interface between the lamellipodial actin meshwork and the membrane. - Information by UniProt
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Database links
- Entrez Gene: 10163 Human
- Entrez Gene: 242687 Mouse
- Entrez Gene: 313024 Rat
- Omim: 605875 Human
- SwissProt: Q9Y6W5 Human
- SwissProt: Q8BH43 Mouse
- Unigene: 469244 Human
- Unigene: 590909 Human
see all -
Alternative names
- DICTYOSTELIUM antibody
- dJ393P12.2 antibody
- IMD2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASF2/WAVE2 antibody (ab219812)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung tissue sections labeling WASF2/WAVE2 with ab219812 at a 1/50 dilution.
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Western blot - Anti-WASF2/WAVE2 antibody (ab219812)Anti-WASF2/WAVE2 antibody (ab219812) at 1/500 dilution + K562 cell lysate
Predicted band size: 54 kDa
Protocols
Datasheets and documents
References (0)
ab219812 has not yet been referenced specifically in any publications.
