Overview

  • Product name

    Anti-WASH1 antibody - C-terminal
  • Description

    Rabbit polyclonal to WASH1 - C-terminal
  • Host species

    Rabbit
  • Specificity

    The immunogen has low homology compared with other WASP family members.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide coupled to a carrier protein, corresponding to C terminal amino acids of Human WASH1 (UniProt: A8K0Z3).

  • Positive control

    • Jurkat whole cell lysate (ab7899); Chick DRG neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab157592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF 1/200.

Target

Images

  • Immunocytochemical analysis of Chick DRG neurons labeling WASH1 with ab157592 at 1/200 dilution (green). Left image shows WASH1 labeling compared to F-actin staining (red).
  • Lane 1 : Anti-WASH1 antibody - C-terminal (ab157592) at 1/250 dilution
    Lanes 2-3 : Anti-WASH1 antibody - C-terminal (ab157592) at 1/1000 dilution

    Lanes 1-2 : Jurkat cells
    Lane 3 : Jurkat cells with blocking peptide

    Predicted band size: 50 kDa

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 27 2019

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