Anti-WASH1 antibody - C-terminal (ab157592)
Key features and details
- Rabbit polyclonal to WASH1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-WASH1 antibody - C-terminal -
Description
Rabbit polyclonal to WASH1 - C-terminal -
Host species
Rabbit -
Specificity
The immunogen has low homology compared with other WASP family members. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Chicken, Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide coupled to a carrier protein, corresponding to C terminal amino acids of Human WASH1 (UniProt: A8K0Z3).
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Positive control
- Jurkat whole cell lysate (ab7899); Chick DRG neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 50 kDa.
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ICC/IF |
1/200.
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Notes |
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WB
1/1000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/200. |
Target
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Function
Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. -
Sequence similarities
Belongs to the WASH1 family.
Contains 1 WH2 domain. -
Cellular localization
Early endosome membrane. Recycling endosome membrane. Localization to the endodome membrane is mediated via its interaction with FAM21. - Information by UniProt
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Database links
- Entrez Gene: 418145 Chicken
- Entrez Gene: 100287171 Human
- Entrez Gene: 68767 Human
- Entrez Gene: 367328 Rat
- Omim: 613632 Human
- SwissProt: Q5ZKA6 Chicken
- SwissProt: A8K0Z3 Human
- SwissProt: Q8VDD8 Mouse
see all -
Alternative names
- CXYorf1-like protein on chromosome 9 antibody
- Protein FAM39E antibody
- WAS protein family homolog 1 antibody
see all
Images
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Immunocytochemical analysis of Chick DRG neurons labeling WASH1 with ab157592 at 1/200 dilution (green). Left image shows WASH1 labeling compared to F-actin staining (red).
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Lane 1 : Anti-WASH1 antibody - C-terminal (ab157592) at 1/250 dilution
Lanes 2-3 : Anti-WASH1 antibody - C-terminal (ab157592) at 1/1000 dilution
Lanes 1-2 : Jurkat cells
Lane 3 : Jurkat cells with blocking peptide
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab157592 has been referenced in 7 publications.
- Hong Y et al. WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B. PeerJ 10:e13499 (2022). PubMed: 35855902
- Guo M et al. Loss of miR-637 promotes cancer cell stemness via WASH/IL-8 pathway and serves as a novel prognostic marker in esophageal squamous cell carcinoma. Biomark Res 10:77 (2022). PubMed: 36329557
- Noguchi Y et al. Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling. iScience 24:102724 (2021). PubMed: 34337357
- Farina F et al. Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis. EMBO J 38:N/A (2019). PubMed: 31015335
- Farina F et al. The centrosome is an actin-organizing centre. Nat Cell Biol 18:65-75 (2016). PubMed: 26655833
- Vanhoutte D et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB . PubMed: 27669143
- Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). PubMed: 25987849