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Signal Transduction Adapters Cytoplasmic
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Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
  • Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)

Key features and details

  • Rabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human WASP/Wiskott-Aldrich syndrome protein (ab152802)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-WASP/Wiskott-Aldrich syndrome protein antibody
    See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
  • Description

    Rabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human WASP/Wiskott-Aldrich syndrome protein aa 60-250.
    Sequence:

    GAEHWTKEHCG AVCFVKDNPQ KSYFIRLYGL QAGRLLWEQE LYSQLVYSTP TPFFHTFAGD DCQAGLNFAD EDEAQAFRAL VQEKIQKRNQ RQSGDRRQLP PPPTPANEER RGGLPPLPLH PGGDQGGPPV GPLSLGLATV DIQNPDITSS RYRGLPAPGP SPADKKRSGK KKISKADIGA PSGFKHVSHV


    Database link: P42768
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HL60, THP1 cell lysate
  • General notes

     This product was previously labelled as WASP

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • THP-1 whole cell lysate (ab7913)
    • HL-60 whole cell lysate (ab7914)
  • Recombinant Protein

    • Recombinant Human WASP/Wiskott-Aldrich syndrome protein (ab152802)
  • Related Products

    • Recombinant Human N WASP protein (ab132277)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 53 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/500 - 1/2000. Predicted molecular weight: 53 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
  • Tissue specificity

    Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
  • Involvement in disease

    Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
    Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
    Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
  • Sequence similarities

    Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 1 WH2 domain.
  • Domain

    The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
    The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P42768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7454 Human
    • Entrez Gene: 317371 Rat
    • Omim: 300392 Human
    • SwissProt: P42768 Human
    • Unigene: 2157 Human
    • Alternative names

      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling WASP/Wiskott-Aldrich syndrome protein with ab180816 at 1/100. Magnification: 200x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling WASP/Wiskott-Aldrich syndrome protein with ab180816 at 1/100. Magnification: 200x.

    • Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
      Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816)
      All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab180816) at 1/500 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : THP1 cell lysate

      Predicted band size: 53 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab180816? Please let us know so that we can cite the reference in this datasheet.

    ab180816 has not yet been referenced specifically in any publications.

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