Key features and details
- Rabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WASP/Wiskott-Aldrich syndrome protein antibody
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
DescriptionRabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment (His-T7-tag) corresponding to Human WASP/Wiskott-Aldrich syndrome protein aa 39-251. Expressed in E.coli. N-terminal tags.
LGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLY GLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFR ALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGP PVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADI GAPSGFKHVSHVG
Database link: P42768
- WB: HL-60 cell lysate; Recombinant human WASP/Wiskott-Aldrich syndrome protein. IHC-P: Human spleen tissue.
This product was previously labelled as WASP
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatograph.
Our Abpromise guarantee covers the use of ab233216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.
DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
Formalin-fixed, paraffin-embedded human spleen tissue stained for WASP/Wiskott-Aldrich syndrome protein using ab233216 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216) at 1 µg/ml + HL-60 (human promyelocytic leukemia cell line) cell lysate
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216) at 2 µg/ml + Recombinant human WASP/Wiskott-Aldrich syndrome protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233216 has not yet been referenced specifically in any publications.