Product nameAnti-WASP/Wiskott-Aldrich syndrome protein antibody
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
DescriptionGoat polyclonal to WASP/Wiskott-Aldrich syndrome protein
This antibody reacts to the Wiskott-Aldrich Syndrome Protein. It is not expected to cross-react with N WASP/Wiskott-Aldrich syndrome protein (WASL).
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- U937 and MOLT4 cell lysates.
This product was previously labelled as WASP
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab28769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.
DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab28769) at 0.03 µg/ml + U937 cell lysate (total protein/per lane) at 30 µg/ml
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53-65 kDa why is the actual band size different from the predicted?
ab28769 has not yet been referenced specifically in any publications.