Product nameAnti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
DescriptionRabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human WASP/Wiskott-Aldrich syndrome protein aa 450-550 (C terminal). The exact sequence is proprietary.
- TF-1 and U937 cell lysates. Human spleen tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as WASP
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.
DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/50000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab75830 at 1/100 dilution staining WASP/Wiskott-Aldrich syndrome protein in paraffin-embedded human spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometry analysis of permeabilized U937 cells, staining WASP/Wiskott-Aldrich syndrome protein (red) with ab75830 at 1/10 dilution. Rabbit IgG was used as negative control (green).
ab75830 has not yet been referenced specifically in any publications.