RabMAb

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

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Overview

  • Product name

    Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]
    See all WASP/Wiskott-Aldrich syndrome protein primary antibodies

  • Description

    Rabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human WASP/Wiskott-Aldrich syndrome protein aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • TF-1 and U937 cell lysates. Human spleen tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as WASP

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP2541Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.

    • Tissue specificity

      Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

    • Involvement in disease

      Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.

    • Sequence similarities

      Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.

    • Domain

      The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      • Alternative names

        • Eczema thrombocytopenia antibody
        • IMD2 antibody
        • SCNX antibody
        • THC antibody
        • THC1 antibody
        • Thrombocytopenia 1 (X linked) antibody
        • U42471 antibody
        • Was antibody
        • WASp antibody
        • WASP_HUMAN antibody
        • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
        • Wiskott Aldrich syndrome antibody
        • Wiskott Aldrich syndrome protein antibody
        • Wiskott-Aldrich syndrome protein antibody
        see all

      Images

      • Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
        Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
        All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/50000 dilution

        Lane 1 : TF-1 cell lysate
        Lane 2 : U937 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 53 kDa
        Observed band size: 60 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

        ab75830 at 1/100 dilution staining WASP/Wiskott-Aldrich syndrome protein in paraffin-embedded human spleen tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
        Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

        Flow cytometry analysis of permeabilized U937 cells, staining WASP/Wiskott-Aldrich syndrome protein (red) with ab75830 at 1/10 dilution. Rabbit IgG was used as negative control (green).

      References

      ab75830 has not yet been referenced specifically in any publications.

      Publishing research using ab75830? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Submit a review Submit a question

      Question

      Dear Sir or Madam!

      I would like to kindly ask some questions about your product:
      Code: ab75830
      Product name: Anti-WASP antibody [EP2541Y]
      Lot:892315

      I have read the datasheet of this product and you wrote this antibody is unsuitable for Flow cytometry. I would like to kindly ask you why you say that. Did you make some tests with this antibody and relying upon these findings you wrote it is unsuitable for Flow cytometry or you did not try this product in Flow cytometry analysis?
      I tried this WASP antibody by Flow cytometer and I got prosperous results.

      Please, send me more details with this problem, because I would like to use this antibody by flow cytometer but I read there are no references.

      (I also tried this product in Western blot analysis and I got similar results which I found by Flow cytometry. On the basis of this finding I thought it is suitable for Flow cytometry.)

      You wrote the concentration is not determined. But the concentration values are important data in Flow cytometry analysis. So I would like to kindly ask you about the concentration value in ug/ml.

      I would like to say thank you in advance.
      Yours faithfully,

      Read More
      Answer

      Thank you for your enquiry.

      I have contacted our laboratory source for this antibody. They have confirmed that the antibody was only tested once with one protocol in flow cytometry. In order to be honest with our customers, we have therefore stated does not work on the datasheet and we cannot guarantee it will work in flow cytometry until it is successfully tested. I am sorry we have no other flow cytometry data on this occasion. In this case, with a more thoroughly optimized protocol, it may be that the antibody will work.

      I am pleased to hear it has worked in your flow cytometry experiments. We like to encourage all of our customers to submit an Abreview via the online product datasheet, and this would be particularly beneficial in this case. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

      The originator of the antibody is also going to retest the antibody in this application. This may take some time, but I will be pleased to forward any additional data that we are sent.

      Should any further data we receive from the originator or in an Abreview from a customer indicate that this antibody will work in flow cytometry, we will be able to amend the datasheet to say successfully tested in flow cytometry.

      I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

      Read More

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