Product nameAnti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
DescriptionRabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein (phospho Y290)
ab59278 detects endogenous levels of WASP/Wiskott-Aldrich syndrome protein only when phosphorylated at tyrosine 290.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human WASP/Wiskott-Aldrich syndrome protein.
- WB: Extracts from HepG2 cells. IHC-P: Human brain tissue.
This product was previously labelled as WASP
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab59278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.
DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59278 at a dilution of 1/50.
Left image: no immunising peptide.
Right hand image: with immunising peptide.
All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (ab59278) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunising peptide
Lane 2 : Extracts from HepG2 cells with immunising peptide
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Liu C et al. N-wasp is essential for the negative regulation of B cell receptor signaling. PLoS Biol 11:e1001704 (2013). Flow Cyt, ICC/IF ; Mouse, Human . Read more (PubMed: 24223520) »
- Malet-Engra G et al. CIP4 controls CCL19-driven cell steering and chemotaxis in chronic lymphocytic leukemia. Cancer Res 73:3412-24 (2013). WB ; Human . Read more (PubMed: 23644527) »