Recombinant Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-WAVE 1 antibody [EPR15021] - N-terminal
See all WAVE 1 primary antibodies -
Description
Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, 293T and K562 cell lysate. K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15021 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).
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Notes |
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. -
Tissue specificity
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood. -
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain. -
Domain
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain. -
Cellular localization
Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas. - Information by UniProt
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Database links
- Entrez Gene: 8936 Human
- Entrez Gene: 83767 Mouse
- Entrez Gene: 294568 Rat
- Omim: 605035 Human
- SwissProt: Q92558 Human
- SwissProt: Q8R5H6 Mouse
- SwissProt: Q5BJU7 Rat
- Unigene: 75850 Human
see all -
Alternative names
- WASP family, verprolin homology domain-containing protein 1 antibody
- WASP family member 1 antibody
- FLJ31482 antibody
see all
Images
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All lanes : Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab185546 has not yet been referenced specifically in any publications.