Overview

  • Product name

    Anti-WAVE 1 antibody [EPR15021] - N-terminal
    See all WAVE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human WAVE 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q92558

  • Positive control

    • U87-MG, 293T and K562 cell lysate. K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).

Target

  • Function

    Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
  • Tissue specificity

    Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
  • Sequence similarities

    Belongs to the SCAR/WAVE family.
    Contains 1 WH2 domain.
  • Domain

    Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
  • Cellular localization

    Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
  • Information by UniProt
  • Database links

  • Alternative names

    • WASP family, verprolin homology domain-containing protein 1 antibody
    • WASP family member 1 antibody
    • FLJ31482 antibody
    • homology of dictyostelium scar 1 antibody
    • KIAA0269 antibody
    • Protein WAVE-1 antibody
    • Protein WAVE1 antibody
    • scar, Dictyostelium, homology of, 1 antibody
    • SCAR1 antibody
    • Similar to a plant extensin like protein antibody
    • Verprolin homology domain containing protein 1 antibody
    • Verprolin homology domain-containing protein 1 antibody
    • WAS protein family, member 1 antibody
    • Wasf1 antibody
    • WASF1_HUMAN antibody
    • WASL antibody
    • WASP family 1 antibody
    • WASP family protein member 1 antibody
    • WASP family, verprolin homology domain-containing protein antibody
    • WAVE antibody
    • WAVE1 antibody
    • Wiskott Aldrich syndrome protein family member 1 antibody
    • Wiskott-Aldrich syndrome protein family member 1 antibody
    see all

Images

  • All lanes : Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

References

ab185546 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185546.
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