Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-WAVE 1 antibody [EPR15021] - N-terminal
See all WAVE 1 primary antibodies
DescriptionRabbit monoclonal [EPR15021] to WAVE 1 - N-terminal
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human WAVE 1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q92558
- U87-MG, 293T and K562 cell lysate. K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).|
FunctionDownstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Tissue specificityHighly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Sequence similaritiesBelongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
DomainBinds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localizationCytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
- Information by UniProt
- WASP family, verprolin homology domain-containing protein 1 antibody
- WASP family member 1 antibody
- FLJ31482 antibody
All lanes : Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
ab185546 has not yet been referenced specifically in any publications.