Anti-WBP11 antibody (ab204361)
Key features and details
- Rabbit polyclonal to WBP11
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WBP11 antibody
See all WBP11 primary antibodies -
Description
Rabbit polyclonal to WBP11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human WBP11 aa 550 to the C-terminus (internal sequence).
Database link: Q9Y2W2 -
Positive control
- WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate; IHC-P: Human heart muscle tissue, ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 70 kDa.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 70 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity. -
Tissue specificity
Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung. -
Cellular localization
Nucleus. Cytoplasm. Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin. - Information by UniProt
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Database links
- Entrez Gene: 51729 Human
- Entrez Gene: 60321 Mouse
- Entrez Gene: 103689936 Rat
- Entrez Gene: 297695 Rat
- Entrez Gene: 402950 Zebrafish
- SwissProt: Q9Y2W2 Human
- SwissProt: Q923D5 Mouse
- SwissProt: Q5PQQ2 Rat
see all -
Alternative names
- DKFZp779M1063 antibody
- MGC94547 antibody
- Npw38 binding protein antibody
see all
Images
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling WBP11 in the nucleoplasm with ab204361 at 2 µg/ml.
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Immunohistochemical analysis of human heart muscle tissue labeling WBP11 in the nucleus of myocytes with ab204361 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-WBP11 antibody (ab204361) at 0.4 µg/ml + NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Predicted band size: 70 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204361 has not yet been referenced specifically in any publications.