Anti-WBP11 antibody (ab241432)
Key features and details
- Rabbit polyclonal to WBP11
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WBP11 antibody
See all WBP11 primary antibodies -
Description
Rabbit polyclonal to WBP11 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human WBP11 aa 175-225. The exact sequence is proprietary.
Database link: Q9Y2W2 -
Positive control
- IP: WBP11 IP in HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to WBP11 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab241432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity. -
Tissue specificity
Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung. -
Cellular localization
Nucleus. Cytoplasm. Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin. - Information by UniProt
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Database links
- Entrez Gene: 51729 Human
- Entrez Gene: 60321 Mouse
- Entrez Gene: 103689936 Rat
- Entrez Gene: 297695 Rat
- SwissProt: Q9Y2W2 Human
- SwissProt: Q923D5 Mouse
- SwissProt: Q5PQQ2 Rat
- Unigene: 655138 Human
see all -
Alternative names
- DKFZp779M1063 antibody
- MGC94547 antibody
- Npw38 binding protein antibody
see all
Images
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Immunoprecipitation - Anti-WBP11 antibody (ab241432)
WBP11 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241432 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab241432 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 3 mi nutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241432 has not yet been referenced specifically in any publications.
