Overview

  • Product name

  • Description

    Rabbit polyclonal to WDFY3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)

  • Positive control

    • Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified using an epitope specific to WDFY3 immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    WDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
  • Cellular localization

    Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
  • Database links

  • Alternative names

    • Alfy antibody
    • Autophagy linked FYVE protein antibody
    • KIAA0993 antibody
    • MGC16461 antibody
    • WD repeat and FYVE domain containing 3 antibody
    • ZFYVE25 antibody
    see all

Images

  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • ab84888 staining WDFY3 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

References

ab84888 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you very much for your interest in our products.


To our knowledge, ab84888 has not been tested in WB. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa (Cervical Epithelium Carcinoma))
Specification
HeLa (Cervical Epithelium Carcinoma)
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

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Verified customer

Submitted Sep 08 2010

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