Key features and details
- Rabbit polyclonal to WDFY3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDFY3 antibody
See all WDFY3 primary antibodies
DescriptionRabbit polyclonal to WDFY3
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified using an epitope specific to WDFY3 immobilized on solid support
Our Abpromise guarantee covers the use of ab84888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
RelevanceWDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
Cellular localizationMembrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
- Alfy antibody
- Autophagy linked FYVE protein antibody
- KIAA0993 antibody
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
ab84888 has not yet been referenced specifically in any publications.