Key features and details
- Rabbit polyclonal to WDFY3 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-WDFY3 antibody - N-terminal
See all WDFY3 primary antibodies
DescriptionRabbit polyclonal to WDFY3 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- ICC/IF: RKO cells. WB: Rat brain lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 395 kDa.|
RelevanceWDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
Cellular localizationMembrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
- Alfy antibody
- Autophagy linked FYVE protein antibody
- KIAA0993 antibody
Anti-WDFY3 antibody - N-terminal (ab240917) at 1/1000 dilution (1 hour at RT) + Rat brain lysate at 15 µg
Goat Anti-Rabbit HRP for 60 min at RT at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 395 kDa
Exposure time: 6 minutes
Block: 5% Skimmed milk in 1X TBST.
Immunocytochemistry/Immunofluorescence analysis of RKO (human colon carcinoma cell line ) cells labeling WDFY3 using ab240917 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240917 (D) Composite.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240917 has not yet been referenced specifically in any publications.