Key features and details
- Rabbit polyclonal to WDHD1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDHD1 antibody
See all WDHD1 primary antibodies
DescriptionRabbit polyclonal to WDHD1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Orangutan
A synthetic peptide of a region between residues 975 and 1025 of human WDHD1.
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
Cellular localizationNuclear; nucleoplasm.
- Acidic nucleoplasmic DNA binding protein 1 antibody
- And 1 antibody
- And1 antibody
Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72429 followed by WB (20% of IP loaded):
Lane 1: ab72429 precipitate
Lane 2: ab72436 precipitate
Lane 3: Control IgG
For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.
Detection: ReliaBLOT® Reagents and Procedures were incorporated followed by chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab72429 has been referenced in 1 publication.
- Wang X et al. Aspirin ameliorates cerebral infarction through regulation of TLR4/NF-?B-mediated endoplasmic reticulum stress in mouse model. Mol Med Rep 17:479-487 (2018). PubMed: 29115440