Overview

  • Product name
    Anti-WDR1 antibody [EPR8793]
    See all WDR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8793] to WDR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide within Human WDR1 aa 300-400. The exact sequence is proprietary.
    Database link: O75083

  • Positive control
    • Raji and U87-MG cell lysates. Human skeletal muscle tissue and endometrial carcinoma tissue. Raji cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8793
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab173574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-WDR1 antibody [EPR8793] (ab173574) at 1/1000 dilution

      Lane 1 : Raji cell lysates
      Lane 2 : U87-MG cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 66 kDa

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    • Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

    References

    This product has been referenced in:
    • Iqbal J  et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). Read more (PubMed: 30054946) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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