Key features and details
- Rabbit polyclonal to WDR11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDR11 antibody
See all WDR11 primary antibodies
DescriptionRabbit polyclonal to WDR11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide, corresponding to a region within amino acids 1174-1224 of Human WDR11, NP_060587.8
- Whole cell lysate from HeLa and 293T cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceWDR11 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. The WDR11 gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus.
Cellular localizationMembrane; Single-pass membrane protein, Cytoplasmic and Nuclear
- Bromodomain and WD repeat containing protein 2 antibody
- Bromodomain and WD repeat domain containing 2 antibody
- Bromodomain- and WD repeat-containing protein 2, formerly antibody
All lanes : Anti-WDR11 antibody (ab93871) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 3 minutes
ab93871 at 1 µg/ml detecting WDR11 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of WDR11
Lane 2: ab93871 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence an with exposure time of 10 seconds
ab93871 has been referenced in 2 publications.
- Navarro Negredo P et al. The WDR11 complex facilitates the tethering of AP-1-derived vesicles. Nat Commun 9:596 (2018). WB, ICC/IF . PubMed: 29426865
- Taylor KE & Mossman KL Cellular Protein WDR11 Interacts with Specific Herpes Simplex Virus Proteins at the trans-Golgi Network To Promote Virus Replication. J Virol 89:9841-52 (2015). PubMed: 26178983