Product nameAnti-WDR25 antibody
DescriptionRabbit polyclonal to WDR25
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to amino acids 12-120 of Human WDR25 (Q64LD2).
- RT4, U-251 MG, Human Plasma, Human Liver and Human Tonsil lysates; Human testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in heart, muscle, testis, ovary, uterus and prostate.
Sequence similaritiesContains 7 WD repeats.
- Information by UniProt
- WD repeat-containing protein 25 antibody
- WDR25 antibody
- WDR25_HUMAN antibody
Immunocytochemistry/immunofluorescence analysis of PC-3 cells labelling WDR25 with ab121170 shows positive staining in the nucleus, but excluded from the nucleoli.
All lanes : Anti-WDR25 antibody (ab121170) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121170 at 1/75 dilution staining WDR25 in Paraffin-emebedded Human testis tissue by Immunohistochemistry.
ab121170 has not yet been referenced specifically in any publications.