Overview

  • Product name

    Anti-WDR3 antibody
  • Description

    Rabbit polyclonal to WDR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human WDR3 aa 225-275. The exact sequence is proprietary. NP_006775.1.
    Sequence:

    EIEDPEEPDPKKIKGSSPGIQDTLEAEDGAFETDEAPEDRILSCRKAGSI M


    Database link: Q9UNX4

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab176817 was affinity purified using an epitope specific to WDR3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 106 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    This gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.
  • Cellular localization

    Nucleus, nucleolus.
  • Database links

  • Alternative names

    • dJ776P7.2 (WD repeat domain 3) antibody
    • FLJ12796 antibody
    • WD repeat containing protein 3 antibody
    • WD repeat domain 3 antibody
    • WDR 3 antibody
    • WDR3 antibody
    see all

Images

  • All lanes : Anti-WDR3 antibody (ab176817) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 106 kDa


    Exposure time: 30 seconds
  • Detection of WDR3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176817 at 3 µg/mg lysate for IP (Lane 1).  For WB detection ab176817 was used at 0.1 µg/ml (Lane 2) represents control IgG IP.   Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab176817 has not yet been referenced specifically in any publications.

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