Key features and details
- Rabbit polyclonal to WDR3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDR3 antibody
DescriptionRabbit polyclonal to WDR3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176817 was affinity purified using an epitope specific to WDR3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176817 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 106 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceThis gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.
Cellular localizationNucleus, nucleolus.
- dJ776P7.2 (WD repeat domain 3) antibody
- FLJ12796 antibody
- WD repeat containing protein 3 antibody
All lanes : Anti-WDR3 antibody (ab176817) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Exposure time: 30 seconds
Detection of WDR3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176817 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176817 was used at 0.1 µg/ml (Lane 2) represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab176817 has not yet been referenced specifically in any publications.