Product nameAnti-WDR33 antibody
See all WDR33 primary antibodies
DescriptionRabbit polyclonal to WDR33
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
Tissue specificityMost highly expressed in testis.
Sequence similaritiesContains 1 collagen-like domain.
Contains 7 WD repeats.
- Information by UniProt
- WD repeat containing protein 33 antibody
- WD repeat domain 33 antibody
- WD repeat-containing protein 33 antibody
All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 146 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72115 has not yet been referenced specifically in any publications.