Overview

  • Product name

  • Description

    Rabbit polyclonal to WDR35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 71 - 120 (SGSVQVVTWN EQYQKLTTSD ENGLIIVWML YKGSWIEEMI NNRNKSVVRS) of Human WDR35 isoform 2 (NP_065830).

  • Positive control

    • Human fetal lung lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 132 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    May promote CASP3 activation and TNF-stimulated apoptosis.
  • Involvement in disease

    Defects in WDR35 are the cause of cranioectodermal dysplasia type 2 (CED2) [MIM:613610]. A disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include short stature, dolichocephaly, craniosynostosis, narrow thorax with pectus excavatum, short limbs, brachydactyly, joint laxity, narrow palpebral fissures, telecanthus with hypertelorism, low-set simple ears, everted lower lip, and short neck. Teeth abnormalities included widely spaced, hypoplastic and fused teeth.
  • Sequence similarities

    Contains 5 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Intraflagellar transport protein 121 homolog antibody
    • KIAA1336 antibody
    • MGC33196 antibody
    • Naofen antibody
    • WD repeat domain 35 antibody
    • WD repeat-containing protein 35 antibody
    • WDR35 antibody
    • WDR35_HUMAN antibody
    see all

Images

  • Anti-WDR35 antibody (ab105557) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size: 132 kDa



    Gel concentration: 6-18%

References

ab105557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8% acrylamide/bis)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Apr 04 2014

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