Product nameAnti-WDR4 antibody
See all WDR4 primary antibodies
DescriptionRabbit polyclonal to WDR4
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human WDR4 aa 362-412. The exact sequence is proprietary. (NP_061139.2)
Database link: P57081
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to WDR4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 45 kDa.|
FunctionRequired for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit.
PathwaytRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Sequence similaritiesBelongs to the WD repeat TRM82 family.
Contains 4 WD repeats.
- Information by UniProt
- OTTHUMP00000109401 antibody
- OTTHUMP00000109402 antibody
- TRM82 antibody
All lanes : Anti-WDR4 antibody (ab241297) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes
WDR4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241297 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241297 at 1 µg/ml.
Lane 1: ab241297 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab241297 has not yet been referenced specifically in any publications.