Overview

  • Product name
    Anti-WDR4 antibody [EPR11052]
    See all WDR4 primary antibodies
  • Description
    Rabbit monoclonal [EPR11052] to WDR4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human WDR4 (P57081).

  • Positive control
    • HepG2, HeLa, A549 and MCF7 cell lysates, Human cervical carcinoma and Human tonsil tissues, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11052
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/50 - 1/2000.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit.
    • Pathway
      tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
    • Sequence similarities
      Belongs to the WD repeat TRM82 family.
      Contains 4 WD repeats.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • OTTHUMP00000109401 antibody
      • OTTHUMP00000109402 antibody
      • TRM82 antibody
      • tRNA (guanine N(7) ) methyltransferase subunit WDR4 antibody
      • tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 antibody
      • WD repeat containing protein 4 antibody
      • WD repeat protein 4 antibody
      • WD repeat protein domain 4 protein isoform 2 antibody
      • WD repeat-containing protein 4 antibody
      • wdr4 antibody
      • WDR4_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling WDR4 with purified ab169526 at 1/2000. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • ab169526 showing +ve staining in Human colonic adenocarcinoma tissue.

    • ab169526 showing +ve staining in Human hepatocellular carcinoma tissue.

    • ab169526 showing +ve staining in Human ovarian carcinoma tissue.

    • ab169526 showing +ve staining in Human normal pancreas tissue.

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling WDR4 with ab169526 antibody at 1/250.
    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling WDR4 with ab169526 antibody at 1/250.
    • All lanes : Anti-WDR4 antibody [EPR11052] (ab169526) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Immunofluorescent analysis of HeLa cells labeling WDR4 with ab169526 at 1/50.
    • Flow Cytometrical analysis of permeabilized HeLa cells with ab169526 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    References

    ab169526 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% BIS-TRIS)
    Loading amount
    30 µg
    Treatment
    no treatment, scrambled siRNA, WDR4 siRNA (72 hours)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

    Abcam user community

    Verified customer

    Submitted Jul 28 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    No
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 18°C
    Fixative
    Methanol

    Dr. Clare Logan

    Verified customer

    Submitted Jun 24 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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