Key features and details
- Rabbit polyclonal to WDR42A
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDR42A antibody
See all WDR42A primary antibodies
DescriptionRabbit polyclonal to WDR42A
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
- HeLa and 293T whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176809 was affinity purified using an epitope specific to WDR42A immobilized on solid support.
Our Abpromise guarantee covers the use of ab176809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 67 kDa.|
RelevanceThis gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. The protein contains 7 WD repeats. There are two isoforms produced by alternative splicing.
- DCAF8 antibody
- DDB1 and CUL4 associated factor 8 antibody
- DKFZp781G1096 antibody
All lanes : Anti-WDR42A antibody (ab176809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 3 seconds
Detection of WDR42A in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176809 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab176809 has not yet been referenced specifically in any publications.