Product nameAnti-WDR43 antibody - C-terminal
DescriptionRabbit polyclonal to WDR43 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human WDR43 aa 643-673 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q15061
- Jurkat and HepG2 cell lysate; Human cervical carcinoma tissue; HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab174906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 75 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the UTP5 family.
Contains 6 WD repeats.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- KIAA0007 antibody
- NET12 antibody
- UTP5 antibody
All lanes : Anti-WDR43 antibody - C-terminal (ab174906) at 1/100 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 75 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human carevical carcinoma tissue labeling WDR43 with ab174906 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells labeling EDR43 with ab174906 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab174906 has not yet been referenced specifically in any publications.