Overview

  • Product name
  • Description
    Mouse polyclonal to WDR45
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis, Zebrafish
  • Immunogen

    Full length protein, corresponding to amino acids 1-360 of Human WDR45.

  • Positive control
    • WDR45 transfected 293T cell line; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    WDR45 is a member of the WD repeat protein family. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.
  • Database links
  • Alternative names
    • JM5 antibody
    • NBIA4 antibody
    • WD repeat containing protein 45 antibody
    • WD repeat domain 45 antibody
    • WD repeat domain phosphoinositide interacting protein 4 antibody
    • WD repeat domain, X linked 1 antibody
    • WD45 repeat protein interacting with phosphoinositides 4 antibody
    • WDRX1 antibody
    • WDRXI4 antibody
    • WIPI 4 antibody
    • WIPI-4 antibody
    • WIPI4 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells, labeling WDR45 with ab168532 at 10µg/ml.
  • All lanes : Anti-WDR45 antibody (ab168532) at 1 µg/ml

    Lane 1 : WDR45 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 39 kDa

References

ab168532 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12)
Permeabilization
Yes - 0.1% Triton
Specification
C2C12
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Victoria Barratt

Verified customer

Submitted Jun 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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