Key features and details
- Rabbit polyclonal to WDR45
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDR45 antibody
See all WDR45 primary antibodies
DescriptionRabbit polyclonal to WDR45
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human WDR45 aa 312-322 (C terminal).
Database link: Q9Y484
- ICC/IF: HeLa cells. WB: HEK-293Trap and HeLa cell lysats.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceWDR45 is a member of the WD repeat protein family. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.
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4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR45 (Green) using ab240905 at 1/100 dilution for 60 minutes at RT. Anti-Rabbit ATTO 488 at 1:200 dilution was used as the secondary antibody for 60 minutes at RT. Phalloidin Texas Red F-Actin stain was used as the counterstain at 1/1000 dilution for 60 minutes at RT. The nuclear counterstain was DAPI (Blue) for 5 minutes at RT. Localization: Cytoplasm . Magnification: 60X.
A: DAPI, B: Actin, C: WDR45, D: Composite.
All lanes : Anti-WDR45 antibody (ab240905) at 1/1000 dilution (1 hour at RT)
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293Trap whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Exposure time: 6 minutes
Block: 5% Skim Milk in 1X TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240905 has not yet been referenced specifically in any publications.