Product nameAnti-WDR45 antibody
See all WDR45 primary antibodies
DescriptionRabbit polyclonal to WDR45
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human WDR45 aa 312-322 (C terminal).
Database link: Q9Y484
- ICC/IF: HeLa cells. WB: HEK-293Trap and HeLa cell lysats.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceWDR45 is a member of the WD repeat protein family. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.
- JM5 antibody
- NBIA4 antibody
- WD repeat containing protein 45 antibody
4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR45 (Green) using ab240905 at 1/100 dilution for 60 minutes at RT. Anti-Rabbit ATTO 488 at 1:200 dilution was used as the secondary antibody for 60 minutes at RT. Phalloidin Texas Red F-Actin stain was used as the counterstain at 1/1000 dilution for 60 minutes at RT. The nuclear counterstain was DAPI (Blue) for 5 minutes at RT. Localization: Cytoplasm . Magnification: 60X.
A: DAPI, B: Actin, C: WDR45, D: Composite.
All lanes : Anti-WDR45 antibody (ab240905) at 1/1000 dilution (1 hour at RT)
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293Trap whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Exposure time: 6 minutes
Block: 5% Skim Milk in 1X TBST.
ab240905 has not yet been referenced specifically in any publications.