Overview

  • Product name
    Anti-WDR5 antibody [2C2]
    See all WDR5 primary antibodies
  • Description
    Mouse monoclonal [2C2] to WDR5
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, ChIP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 20864036
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
  • Sequence similarities
    Belongs to the WD repeat WDR5/wds family.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410008O07Rik antibody
    • AA408785 antibody
    • AA960360 antibody
    • BIG 3 antibody
    • Big antibody
    • BIG3 antibody
    • BMP2 induced 3 kb gene protein antibody
    • BMP2 induced gene, 3-KB antibody
    • BMP2-induced 3-kb gene protein antibody
    • MGC114572 antibody
    • OTTHUMP00000162494 antibody
    • SWD 3 antibody
    • SWD3 antibody
    • SWD3 Set1c WD40 repeat protein homolog antibody
    • WD repeat containing protein 5 antibody
    • WD repeat domain 5 antibody
    • WD repeat protein 5 antibody
    • WD repeat-containing protein 5 antibody
    • WD repeat-containing protein BIG-3 antibody
    • WDR 5 antibody
    • Wdr5 antibody
    • WDR5_HUMAN antibody
    see all

Images

  • WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF. 

    This image was generated using the ascites version of the product.

  • Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate

    Predicted band size: 36 kDa



    This image was generated using the ascites version of the product.

References

This product has been referenced in:
  • Rojas A  et al. Mll-COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblasts. J Cell Physiol 234:6244-6253 (2019). Read more (PubMed: 30256410) »
  • He W  et al. Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinoma. Oncol Lett 17:103-112 (2019). Read more (PubMed: 30655744) »
See all 27 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22512.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

DISCOUNT CODE: ************
Expiration date: ***********

I am very pleased to hear you would like to accept our offer and test ab56919 in mouse samples. This code will give you 1 freePRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab56919.

To our knowledge, ab56919 has not been tested in mouse samples however based on sequence homology, I believe there isa very goodchance that it will work. Therefore, I can offer a discount off a future purchase if you buy ab56919 now, test it in mouse samples and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab56919 in mouse. I will then send a discount code. This code must be issued before purchasing ab56919 so please wait for my reply before ordering.

2. Purchase ab56919either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab56919 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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