Product nameAnti-WDR5 antibody
See all WDR5 primary antibodies
DescriptionRabbit polyclonal to WDR5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human WDR5 aa 1-334.
MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSS VKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSN LLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFD ESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDT ASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLK TYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGH TDVVISTACHPTENIIASAALENDKTIKLWKSDC
Database link: P61964
- WB: 293T, 22RV-1, BT474 and HeLa cell lysates; IHC: Human kidney.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 37 kDa.|
FunctionContributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
Sequence similaritiesBelongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats.
- Information by UniProt
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
Paraffin-embedded human kidney cancer tissue stained for WDR5 using ab187178 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-WDR5 antibody (ab187178) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 22RV-1 cell lysate
Lane 3 : BT474 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 37 kDa
Blocking buffer: 3% nonfat dry milk in TBST.
ab187178 has not yet been referenced specifically in any publications.