Western blot abreview for Anti-WDR5 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Kidney)
Loading amount
40 µg
Specification
Kidney
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: santa cruz Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 38 kDa Non-specific: 70 kDa

Additional data

Abcam response
Thank you for your valuable feedback. We can suggest the extra band at 70 kDa is the correct size for a dimer of the WDR5 protein, or may be non specific. We would welcome any further feedback from customers regarding their western bot results from this antibody.

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Verified customer

Submitted Dec 08 2011

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