Western blot abreview for Anti-WDR5 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
40 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (10-20%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL prime
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 90 kDa

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Submitted Apr 24 2012

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