Recombinant Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11350] to WDR5 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-WDR5 antibody [EPR11350] - C-terminal
See all WDR5 primary antibodies -
Description
Rabbit monoclonal [EPR11350] to WDR5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human WDR5 aa 300 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P61964 -
Positive control
- HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells. RAW264.7 and Mouse spleen, rat spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11350 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
Sequence similarities
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11091 Human
- Entrez Gene: 140858 Mouse
- Entrez Gene: 362093 Rat
- Omim: 609012 Human
- SwissProt: P61964 Human
- SwissProt: P61965 Mouse
- SwissProt: Q498M4 Rat
- Unigene: 397638 Human
see all -
Alternative names
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
Images
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All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa -
All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands. -
Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).
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Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab178410 has been referenced in 9 publications.
- Zhang C et al. Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive role. Cell Death Dis 13:532 (2022). PubMed: 35668069
- Yuan J et al. Inhibition of Wdr5 Attenuates Ang-II-Induced Fibroblast-to-Myofibroblast Transition in Cardiac Fibrosis by Regulating Mdm2/P53/P21 Pathway. Biomolecules 12:N/A (2022). PubMed: 36358925
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095
- Zhao J et al. Structural insights into the recognition of histone H3Q5 serotonylation by WDR5. Sci Adv 7:N/A (2021). PubMed: 34144982
- Chen T et al. WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways. Mol Ther 29:2134-2150 (2021). PubMed: 33601056
- Feng Z et al. Mixed Lineage Leukemia 1 Promoted Neuron Apoptosis in Ischemic Penumbra via Regulating ASK-1/TNF-a Complex. Front Neuroanat 14:36 (2020). PubMed: 32792914
- Cui Z et al. Effect of high WDR5 expression on the hepatocellular carcinoma prognosis. Oncol Lett 15:7864-7870 (2018). PubMed: 29731905
- Hoshii T et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell 172:1007-1021.e17 (2018). PubMed: 29474905
- Barbieri I et al. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature 552:126-131 (2017). PubMed: 29186125