Overview

  • Product name
    Anti-WDR5 antibody [EPR11350] - C-terminal
    See all WDR5 primary antibodies
  • Description
    Rabbit monoclonal [EPR11350] to WDR5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WDR5 aa 300 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P61964

  • Positive control
    • HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
    • Sequence similarities
      Belongs to the WD repeat WDR5/wds family.
      Contains 7 WD repeats.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2410008O07Rik antibody
      • AA408785 antibody
      • AA960360 antibody
      • BIG 3 antibody
      • Big antibody
      • BIG3 antibody
      • BMP2 induced 3 kb gene protein antibody
      • BMP2 induced gene, 3-KB antibody
      • BMP2-induced 3-kb gene protein antibody
      • MGC114572 antibody
      • OTTHUMP00000162494 antibody
      • SWD 3 antibody
      • SWD3 antibody
      • SWD3 Set1c WD40 repeat protein homolog antibody
      • WD repeat containing protein 5 antibody
      • WD repeat domain 5 antibody
      • WD repeat protein 5 antibody
      • WD repeat-containing protein 5 antibody
      • WD repeat-containing protein BIG-3 antibody
      • WDR 5 antibody
      • Wdr5 antibody
      • WDR5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa
      Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.

    References

    This product has been referenced in:
    See 1 Publication for this product

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