Product nameAnti-WDR5 antibody [EPR11350] - C-terminal
See all WDR5 primary antibodies
DescriptionRabbit monoclonal [EPR11350] to WDR5 - C-terminal
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human WDR5 aa 300 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P61964
- HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.|
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionContributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
Sequence similaritiesBelongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats.
- Information by UniProt
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).
Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.