Anti-WDR6 antibody (ab243813)
Key features and details
- Rabbit polyclonal to WDR6
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR6 antibody -
Description
Rabbit polyclonal to WDR6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human WDR6 aa 73-149.
Sequence:EPNGDLDLEAMVAVFGSKGLRVVKISWGQGHFWELWRSGLWNMSDWIWDA RWLEGNIALALGHNSVVLYDPVVGCIL
Database link: Q9NNW5 -
Positive control
- IHC-P: Human pancreas tissue. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab243813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Relevance
WDR6 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is ubiquitously expressed in adult and fetal tissues. -
Database links
- Entrez Gene: 11180 Human
- Omim: 606031 Human
- SwissProt: Q3MIT1 Human
- SwissProt: Q6AZD6 Human
- SwissProt: Q6PKC6 Human
- SwissProt: Q9NNW5 Human
- Unigene: 31387 Human
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Alternative names
- FLJ10218 antibody
- MGC126756 antibody
- WD repeat domain 6 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-WDR6 antibody (ab243813)
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling WDR6 using ab243813 at 4 µg/ml (green) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR6 antibody (ab243813)Formalin-fixed, paraffin-embedded human pancreas tissue stained for WDR6 with ab243813 at a 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab243813 has not yet been referenced specifically in any publications.
