Overview

  • Product name
    Anti-WDR66 antibody [2A6F7]
  • Description
    Mouse monoclonal [2A6F7] to WDR66
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human WDR66 aa 1-250. Expressed in E. Coli.
    Sequence:

    MSDAAEAPRE ATGENGETEM KEEEEPNPNY KEVEDPQQES KDDTIAWRES QEEERKTGEE EGEEEGKEDK KIVMEETEEK AGEVQEKEAS GIQEETTVEP QEVTASMIRL ETQITDSQSI TSGIFPKTQR GSKSKLSLQL EDAETDELLR DLSTQIEFLD LDQISPEEQQ ISSPERQPSG ELEEKTDRMP QDELGQERRD LEPENREEGQ ERRVSDIQSK AGISRESLVS STTEDILFQK DKSTPVYPLT


    Database link: Q8TBY9

  • Positive control
    • Human WDR66 recombinant protein; WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate; HepG2 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    2A6F7
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution

    Lane 1 : Non transfected HEK293 cell lysate
    Lane 2 : WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 130 kDa



    Expected MW is 53.9 kDa

  • Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution + Human WDR66 recombinant protein

    Predicted band size: 130 kDa



    Expected MW is 53.9 kDa

  • Flow cytometric analysis of HepG2 cells labeling WDR66 using ab175369 at 1/200 dilution (green) and negative control (purple).

References

ab175369 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175369.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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