Anti-WDR66 antibody [2A6F7] (ab175369)
Key features and details
- Mouse monoclonal [2A6F7] to WDR66
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-WDR66 antibody [2A6F7] -
Description
Mouse monoclonal [2A6F7] to WDR66 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human WDR66 aa 1-250. Expressed in E. Coli.
Sequence:MSDAAEAPRE ATGENGETEM KEEEEPNPNY KEVEDPQQES KDDTIAWRES QEEERKTGEE EGEEEGKEDK KIVMEETEEK AGEVQEKEAS GIQEETTVEP QEVTASMIRL ETQITDSQSI TSGIFPKTQR GSKSKLSLQL EDAETDELLR DLSTQIEFLD LDQISPEEQQ ISSPERQPSG ELEEKTDRMP QDELGQERRD LEPENREEGQ ERRVSDIQSK AGISRESLVS STTEDILFQK DKSTPVYPLT
Database link: Q8TBY9 -
Positive control
- Human WDR66 recombinant protein; WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate; HepG2 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A6F7 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 130 kDa.
|
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 130 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Relevance
WDR66 contains 9 WD repeats. -
Database links
- Entrez Gene: 144406 Human
- SwissProt: Q8TBY9 Human
- SwissProt: Q5RE88 Orangutan
-
Alternative names
- FLJ39783 antibody
- MGC33630 antibody
- WD repeat domain 66 antibody
- WDR 66 antibody
Images
-
All lanes : Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution
Lane 1 : Non transfected HEK293 cell lysate
Lane 2 : WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 130 kDaExpected MW is 53.9 kDa
-
Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution + Human WDR66 recombinant protein
Predicted band size: 130 kDaExpected MW is 53.9 kDa
-
Flow cytometric analysis of HepG2 cells labeling WDR66 using ab175369 at 1/200 dilution (green) and negative control (purple).
Datasheets and documents
-
Datasheet download
References (0)
ab175369 has not yet been referenced specifically in any publications.