Overview

  • Product name

    Anti-WDR68 antibody [EPR8712]
    See all WDR68 primary antibodies
  • Description

    Rabbit monoclonal [EPR8712] to WDR68
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WDR68 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, BxPC-3, HeLa, 293T, and fetal heart lysates; Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the WD repeat DCAF7 family.
      Contains 4 WD repeats.
    • Cellular localization

      Cytoplasm. Nucleus. Overexpression of DIAHP1 or active RHOA causes translocation from the nucleus to cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AN11 antibody
      • Dcaf7 antibody
      • DCAF7_HUMAN antibody
      • DDB1- and CUL4-associated factor 7 antibody
      • HAN11 antibody
      • Human anthocyanin antibody
      • Petunia antibody
      • Seven WD repeat protein of the AN11 family 1 antibody
      • SWAN 1 antibody
      • WD repeat domain 68 antibody
      • WD repeat protein An11 homolog antibody
      • WD repeat-containing protein 68 antibody
      • WD repeat-containing protein An11 homolog antibody
      • WDR68 antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab138490 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab138490, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-WDR68 antibody [EPR8712] (ab138490) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : HeLa lysate
      Lane 4 : 293T lysate
      Lane 5 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling WDR68 with ab138490 at 1/100 dilution.

    References

    This product has been referenced in:

    • Yu D  et al. A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Nucleic Acids Res 47:4462-4475 (2019). Read more (PubMed: 30864669) »
    • Abu Jhaisha S  et al. DYRK1B mutations associated with metabolic syndrome impair the chaperone-dependent maturation of the kinase domain. Sci Rep 7:6420 (2017). Read more (PubMed: 28743892) »
    See all 2 Publications for this product

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