Overview

  • Product name

    Anti-WDR70 antibody
  • Description

    Rabbit polyclonal to WDR70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 604 and 654 of Human WDR70 (NP_060504.1)

  • Positive control

    • HeLa or 293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the WD repeat GAD-1 family.
    Contains 7 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4833422F06Rik antibody
    • FLJ10233 antibody
    • MGC109151 antibody
    • RGD1309487 antibody
    • WD repeat containing protein 70 antibody
    • WD repeat domain 70 antibody
    • WD repeat-containing protein 70 antibody
    • wdr70 antibody
    • WDR70_HUMAN antibody
    see all

Images

  • All lanes : Anti-WDR70 antibody (ab86366) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 73 kDa


    Exposure time: 30 seconds
  • Detection of WDR70 by Western Blot of Immunprecipitate.
    ab86366 at 1µg/ml staining WDR70 in HeLa whole cell lysate immunoprecipitated using ab86366 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab86366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.5%TritonX
Specification
U2OS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 14 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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