Recombinant Anti-WDR77 antibody [EPR10708(B)] (ab154190)
![Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-467519-anti-wdr77-antibody-epr10708-b-westernblot-human-mouse-rat.jpg "Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10708(B)] to WDR77
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-WDR77 antibody [EPR10708(B)]
See all WDR77 primary antibodies -
Description
Rabbit monoclonal [EPR10708(B)] to WDR77 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast cancer and human testis tissue; ICC: 293T cells; Flow Cyt (intra): HeLa cells. WB: HeLa, HEK-293 whole cell lysate. Mouse kidney and spleen lysate and Rat lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10708(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
For unpurified use at 1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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| IHC-P |
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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| ICC/IF | (1) |
1/50 - 1/250.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt (Intra)
1/30. For unpurified use at 1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
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IHC-P
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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ICC/IF
1/50 - 1/250. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. -
Tissue specificity
Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors. -
Sequence similarities
Contains 5 WD repeats. -
Developmental stage
Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation. -
Cellular localization
Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors. - Information by UniProt
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Database links
- Entrez Gene: 79084 Human
- Entrez Gene: 70465 Mouse
- Entrez Gene: 310769 Rat
- Omim: 611734 Human
- SwissProt: Q9BQA1 Human
- SwissProt: Q99J09 Mouse
- SwissProt: Q4QR85 Rat
- Unigene: 204773 Human
see all -
Alternative names
- 2610312E17Rik antibody
- Androgen receptor cofactor p44 antibody
- C79984 antibody
see all
Images
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Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)All lanes : Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa -
![Immunocytochemistry - Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-15-anti-wdr77-antibody-epr10708-b-immunocytochemistry-293t-human.jpg)
Immunocytochemistry - Anti-WDR77 antibody [EPR10708(B)] (ab154190)Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling WDR77 with purified ab154190 at 1/50 dilution (5.58 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-14-anti-wdr77-antibody-epr10708-b-immunohistochemistry-testis-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-13-anti-wdr77-antibody-epr10708-b-immunohistochemistry-breast-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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![Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-16-anti-wdr77-antibody-epr10708-b-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling WDR77 with purified ab154190 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-WDR77 antibody [EPR10708(B)] (ab154190)](https://www.abcam.com/ps/products/154/ab154190/Images/ab154190-11-benefits-of-recombinant-antibodies.png)
Anti-WDR77 antibody [EPR10708(B)] (ab154190)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab154190 has been referenced in 4 publications.
- Chen M et al. PRMT5 regulates ovarian follicle development by facilitating Wt1 translation. Elife 10:N/A (2021). PubMed: 34448450
- Lu W et al. The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation. Biochem Biophys Res Commun 514:1185-1191 (2019). PubMed: 31103260
- Qi H et al. Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. J Biol Chem 293:17769-17779 (2018). PubMed: 30282801
- Lubyova B et al. PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core. PLoS One 12:e0186982 (2017). WB ; Human . PubMed: 29065155