Name: Anti-WDR77 antibody [EPR10708(B)]
Brand: RabMAb
SKU: ab154190
Rating: 5 (2 reviews)

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Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10708(B)] to WDR77
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-WDR77 antibody [EPR10708(B)]
See all WDR77 primary antibodies
Description

Rabbit monoclonal [EPR10708(B)] to WDR77
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in Human WDR77 (UniProt ID: Q9BQA1).
Positive control
- HeLa, LnCAP, 293 and fetal heart lysates; Human colon and prostate tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10708(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human WDR77 protein (Tagged) (ab226226)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human
All applications	Mouse Rat

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF	(1)	1/100 - 1/250.
Flow Cyt		1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Tissue specificity

Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Sequence similarities

Contains 5 WD repeats.
Developmental stage

Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.
Cellular localization

Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
Target information above from: UniProt accession Q9BQA1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 79084 Human
- Entrez Gene: 70465 Mouse
- Entrez Gene: 310769 Rat
- Omim: 611734 Human
- SwissProt: Q9BQA1 Human
- SwissProt: Q99J09 Mouse
- SwissProt: Q4QR85 Rat
- Unigene: 204773 Human
- Unigene: 5110 Mouse
- Unigene: 14128 Rat
see all
Alternative names
- 2610312E17Rik antibody
- Androgen receptor cofactor p44 antibody
- C79984 antibody
- HKMT1069 antibody
- MEP 50 antibody
- MEP-50 antibody
- MEP50 antibody
- MEP50_HUMAN antibody
- Methylosome protein 50 antibody
- MGC2722 antibody
- Nbla10071 antibody
- p44 antibody
- p44/Mep50 antibody
- RGD1310479 antibody
- RP11 552M11.3 antibody
- WD repeat containing protein 77 antibody
- WD repeat domain 77 antibody
- WD repeat-containing protein 77 antibody
- WDR77 antibody
see all

Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

All lanes : Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : LnCAP cell lysate
Lane 3 : 293T cell lysate
Lane 4 : fetal heart lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling WDR77 with Purified ab154190 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Flow Cytometry - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Overlay histogram showing HeLa cells stained with ab154190 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154190, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (ab154190)This image is courtesy of an Abreview submitted by Melanie Blasius

ab154190 staining WRD77 (green) in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS. Samples were incubated with primary antibody (1/150 in PBS + 5% BSA) for 1 hour at 20°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody. Red - DAPI.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling WDR77 with ab154190 at 1/50 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling WDR77 with ab154190 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue using ab154190 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin embedded Human normal testis tissue using ab154190 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154190 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154190 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab154190? Please let us know so that we can cite the reference in this datasheet.

ab154190 has been referenced in 3 publications.

Lu W et al. The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation. Biochem Biophys Res Commun 514:1185-1191 (2019). PubMed: 31103260
Qi H et al. Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. J Biol Chem 293:17769-17779 (2018). PubMed: 30282801
Lubyova B et al. PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core. PLoS One 12:e0186982 (2017). WB ; Human . PubMed: 29065155

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (U2OS cells)

Specification

U2OS cells

Permeabilization

Yes - PBS+ 0.2% Triton-X 100, 10 minutes at RT

Fixative

Paraformaldehyde

Dr. Melanie Blasius

Verified customer

Submitted Jun 28 2013

Application

Western blot

Loading amount

40 µg

Gel Running Conditions

Reduced Denaturing (NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm)

Sample

Human Cell lysate - whole cell (U2OS cells)

Specification

U2OS cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Melanie Blasius

Verified customer

Submitted May 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (3)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-WDR77 antibody [EPR10708(B)]

Western blot