Overview

  • Product name
    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free
    See all WDR77 primary antibodies
  • Description
    Rabbit monoclonal [EPR10709(2)] to WDR77 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WDR77 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BQA1

  • General notes

    ab251023 is a PBS-only buffer format of ab190361. Please refer to ab190361 for recommended dilutions, protocols, and image data.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab251023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
  • Tissue specificity
    Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
  • Sequence similarities
    Contains 5 WD repeats.
  • Developmental stage
    Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.
  • Cellular localization
    Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610312E17Rik antibody
    • Androgen receptor cofactor p44 antibody
    • C79984 antibody
    • HKMT1069 antibody
    • MEP 50 antibody
    • MEP-50 antibody
    • MEP50 antibody
    • MEP50_HUMAN antibody
    • Methylosome protein 50 antibody
    • MGC2722 antibody
    • Nbla10071 antibody
    • p44 antibody
    • p44/Mep50 antibody
    • RGD1310479 antibody
    • RP11 552M11.3 antibody
    • WD repeat containing protein 77 antibody
    • WD repeat domain 77 antibody
    • WD repeat-containing protein 77 antibody
    • WDR77 antibody
    see all

References

ab251023 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab251023.
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