Key features and details
- Rabbit polyclonal to WDR91
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WDR91 antibody
See all WDR91 primary antibodies
DescriptionRabbit polyclonal to WDR91
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 350 and 400 of human WDR91 (NP_054868.2).
- HeLa whole cell lysate. 293T whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 7 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- HSPC049 antibody
- WD repeat containing protein 91 antibody
- WD repeat domain 91 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-WDR91 antibody (ab80614) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 30 seconds
Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3µg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1µg/ml and detected with chemiluminescence with an exposure time of 10 seconds.
ab80614 has not yet been referenced specifically in any publications.