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Rabbit polyclonal to WDR91
Predicted to work with:
Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 350 and 400 of human WDR91 (NP_054868.2).
HeLa whole cell lysate. 293T whole cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 7 WD repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
WD repeat containing protein 91 antibody
WD repeat domain 91 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR91 antibody (ab80614)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-WDR91 antibody (ab80614)
All lanes : Anti-WDR91 antibody (ab80614) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-WDR91 antibody (ab80614)
Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3µg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1µg/ml and detected with chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"