Overview

  • Product name
    Anti-WDTC1 antibody [EPR12504]
  • Description
    Rabbit monoclonal [EPR12504] to WDTC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human WDTC1. The exact sequence is proprietary.
    Database link: Q8N5D0

  • Positive control
    • HepG2 and A431 cell lysates. Human liver and lung carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12504
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      WDTC1 contains 2 TPR repeats and 7 WD repeats. WDTC1, the ortholog of Drosophila Adipose Gene, associates with human obesity, modulated by MUFA intake. There are six named isoforms.
    • Cellular localization
      Cytosol. Nucleus.
    • Database links
    • Alternative names
      • Adipose homolog antibody
      • ADP antibody
      • DCAF9 antibody
      • DDB1 and CUL4 associated factor 9 antibody
      • KIAA1037 antibody
      • WD and tetratricopeptide repeats 1 antibody
      • WD and tetratricopeptide repeats protein 1 antibody
      see all

    Images

    • Ab174294 staining WDTC1 in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/50 dilution (1.6 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2 μg/ml. An AlexaFluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker, ab195889 was used as a counterstain antibody (1/200, 2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line.

    • All lanes : Anti-WDTC1 antibody [EPR12504] (ab174294) at 1/10000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 76 kDa

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate  immunoprecipitated using ab174294 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling WDTC1 with ab174294 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling WDTC1 with ab174294 at 1/50 dilution.

    References

    This product has been referenced in:
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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