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Recombinant fragment within Human WDTC1. The exact sequence is proprietary.
Database link: Q8N5D0
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab174294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 76 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
Ab174294 staining WDTC1 in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/50 dilution (1.6 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2 μg/ml. An AlexaFluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker, ab195889 was used as a counterstain antibody (1/200, 2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line.
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab174294 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling WDTC1 with ab174294 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling WDTC1 with ab174294 at 1/50 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"