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sdfsfdsfdsfdsdfsdfdsdsdsfdsfdsfsfdquality of protein reagents is critical to ensuring reproducibility and consistency for research. In this webinar, Deborah Moore-Lai briefly introduces a new protein product line and discusses the approach taken at Abcam to ensure batch-to-batch consistency of these proteins.
he Antibody Production team
I've heard about large value orders (LVOs). What does this mean? And how do I inquire about one?
DML: It depends on your research needs and the protein itself. For example, if the protein is intracellular and does not have disulfide bonds or post-translational modifications, a prokaryotic bacterial system will suit your needs. If, on the other hand, your protein is complex and it is secreted, and it has disulfide bonds that you would prefer that the protein is expressed with, then mammalian is likely a better option for you.
DML: My recommendation is don't have endotoxins there in the first place. The lab in Waltham is exclusively mammalian, and so it's very, very clean. The instruments are sanitized weekly with a really deep sodium hydroxide clean to ensure that there's nothing growing in them over the weekend. We do have removal methods if there is endotoxin in the sample, but you take a hit on your protein yield. My recommendation is just to use ultra-pure reagents.
How do you pick which cell-based assay to use for your proteins?
DML: What we do is look at the literature and look for the gold standard assay, and then we proceed accordingly. In terms of difficulty, proliferation assays are the most straightforward for the lab to run and that would be ideal. Sometimes it's chemokines, and obviously, we need to do a chemotaxis assay. We find those are some of the hardest and most difficult assays to run.
We see what our competitors are using for whatever that gold standard assay is, and then we proceed accordingly. And we will also often include a calibrator protein just as a positive control to understand if our protein is behaving properly or if it's something about the assay itself, and the setup of the assay that we need to redesign.
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