Enabling Spatial Proteomic Profiling With Highly Validated Antibodies And Custom Content
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Now available to watch on-demand! Join Abcam and NanoString for talks and discussion!
Description
Join Abcam and NanoString to hear how we are working together to offer GeoMx®-formatted antibodies for use with the GeoMx® Digital Spatial Profiler (DSP). You will hear from scientists from both Abcam and NanoString, explaining the process of spatial proteomic profiling and antibody validation.
Our aims are to:
- Learn how the GeoMx® Digital Spatial Profiler enables high-plex protein and RNA experiments
- Understand the necessity of antibody validation
- Learn NanoString’s process of validating multiplex protein assays
- Appreciate how Abcam validates RabMAb® antibodies in IHC
- Appreciate how an extension of this facilitates QC IHC testing of NanoString conjugates
Confirmed speakers
- Alyssa Rosenbloom, NanoString
- The GeoMx® Digital Spatial Profiler (DSP) enables investigation of high-plex, spatially resolved protein and RNA targets from a single slide-mounted formalin-fixed paraffin-embedded (FFPE) or fresh frozen sample. In this presentation, Alyssa will review NanoString’s robust process to screen, conjugate, and validate oligo-tagged antibodies before their incorporation into commercial panels. Antibodies with poor quality can lead to wasted time and resources, including false positives and non-reproducible results. To enable customers to focus on research, every antibody in NanoString’s commercial panels undergoes rigorous testing to ensure appropriate specificity, sensitivity, and overall performance.
- Simon Renshaw, Abcam
- Abcam offers over 4,000 suitable RabMAb® antibodies, empowering you to customize your GeoMx® DSP Protein Assays. Each of these antibodies undergoes bespoke IHC validation in FFPE tissues, to ensure high specificity and reproducibility. Simon's talk will highlight the process of RabMAb® IHC antibody validation at Abcam, encompassing both pre- and post-DSP barcode conjugation.
Panel discussion
- Gary Geiss, NanoString
- Will Howat, Abcam