Overview

  • Product name

    Anti-Wee1 antibody [EPR20899]
    See all Wee1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20899] to Wee1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Recombinant fragment within Schizosaccharomyces pombe Wee1 aa 1-200. The exact sequence is proprietary.
    Database link: P07527

  • Positive control

    • WB: Fission yeast (Schizosaccharomyces pombe) whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa).

Target

  • Function

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Alternative names

    • DKFZp686I18166 antibody
    • EC 2.7.10.2 antibody
    • FLJ16446 antibody
    • MGC105683 antibody
    • OTTHUMP00000231338 antibody
    • OTTHUMP00000231339 antibody
    • Wee 1 antibody
    • WEE 1 homolog 1 (S. pombe) antibody
    • WEE1 antibody
    • WEE1 homolog (S. pombe) antibody
    • Wee1 homolog antibody
    • WEE1 homolog S. pombe antibody
    • Wee1 like protein kinase antibody
    • Wee1 tyrosine kinase antibody
    • Wee1+ homolog antibody
    • Wee1+ S. pombe homolog antibody
    • WEE1, S. pombe, homolog of antibody
    • WEE1, somatic antibody
    • Wee1-like protein kinase antibody
    • WEE1_HUMAN antibody
    • WEE1A antibody
    • Wee1A kinase antibody
    • WEE1hu antibody
    see all

Images

  • All lanes : Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution

    Lane 1 : Wild-type Schizosaccharomyces pombe whole cell lysate
    Lane 2 : Wee1 knockout Schizosaccharomyces pombe whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 96 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 70 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.

References

ab233540 has not yet been referenced specifically in any publications.

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