Anti-Wee1 (phospho S642) antibody (ab203213)
Key features and details
- Rabbit polyclonal to Wee1 (phospho S642)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Wee1 (phospho S642) antibody
See all Wee1 primary antibodies -
Description
Rabbit polyclonal to Wee1 (phospho S642) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Wee1 aa 600 to the C-terminus (C terminal) (phospho S642) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P30291 -
Positive control
- Human brain glioma tissue; Human liver carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
|
Notes |
---|
IHC-P
1/100 - 1/500. |
Target
-
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7465 Human
- Entrez Gene: 22390 Mouse
- Entrez Gene: 308937 Rat
- Omim: 193525 Human
- SwissProt: P30291 Human
- SwissProt: P47810 Mouse
- SwissProt: Q63802 Rat
- Unigene: 249441 Human
see all -
Alternative names
- DKFZp686I18166 antibody
- EC 2.7.10.2 antibody
- FLJ16446 antibody
see all
Images
-
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human brain glioma tissue labeling Wee1 with ab203213 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
-
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human liver carcinoma tissue labeling Wee1 with ab203213 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab203213 has not yet been referenced specifically in any publications.