Key features and details
- Rabbit polyclonal to Wee1 (phospho S642)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Wee1 (phospho S642) antibody
See all Wee1 primary antibodies
DescriptionRabbit polyclonal to Wee1 (phospho S642)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human brain glioma tissue; Human liver carcinoma tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase.
- Information by UniProt
- DKFZp686I18166 antibody
- EC 220.127.116.11 antibody
- FLJ16446 antibody
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human brain glioma tissue labeling Wee1 with ab203213 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human liver carcinoma tissue labeling Wee1 with ab203213 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
ab203213 has not yet been referenced specifically in any publications.